| 质粒名称: | mVenus C1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 27794 |
| 存储实验室: | Steven Vogel |
|
载体骨架基本信息 |
|
| 载体名称: | pEYFP C1 |
| 空载体大小: | 3984 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mVenus C1,mVenus-C1 |
| 插入大小: | 720 bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Nhe 1(未破坏) |
| 3’克隆位点: | Bam HI(未破坏) |
| 5’测序引物 | CCAAAATCAACGGGACTTTCC |
| 3’测序引物 | CAGGTTCAGGGGGAGGTGTGG |
|
文献引用方法 |
|
| 材料和方法部分: | mVenus C1 was a gift from Steven Vogel (Addgene plasmid # 27794 ; http://n2t.net/addgene:27794 ; RRID:Addgene_27794) |
| 参考文献部分: | Cerulean, Venus, and VenusY67C FRET reference standards. Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS. Biophys J. 2006 Dec 15. 91(12):L99-L101. 10.1529/biophysj.106.096206 PubMed 17040988 |
买家导航
mVenusC1载体基本信息
mVenusC1载体图谱质粒图谱和多克隆位点信息
mVenusC1载体简介
mVenusC1载体序列
LOCUS mVenus C1 4731 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS mVenus C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4731)
AUTHORS Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS
TITLE Cerulean, Venus, and VenusY67C FRET reference standards.
JOURNAL Biophys J. 2006 Dec 15. 91(12):L99-L101.
PUBMED 17040988
REFERENCE 2 (bases 1 to 4731)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4731
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 83..386
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 387..590
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 541..561
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 629..638
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 635..1351
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002; Kremers et al., 2006)"
/label=mVenus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(680..701)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(941..960)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1288..1309
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 1352..1417
/label=MCS
/note="multiple cloning site"
polyA_signal 1541..1662
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1578..1597)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1632..1651
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1669..2124)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1806..1827)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2018..2037
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2151..2255
/gene="bla"
/label=AmpR promoter
promoter 2257..2614
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2280..2300)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2465..2600
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2527..2546
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2649..3443
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2703..2722)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3313..3332
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3631..3650)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3675..3722
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4051..4639
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4540..4559
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
601 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
661 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
721 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctcatctg
781 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctcg gctacggcct
841 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
901 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
961 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
1021 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
1081 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
1141 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
1201 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
1261 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
1321 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
1381 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
1441 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
1501 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
1561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
1621 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
1681 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
1741 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
1801 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
1861 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
1921 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
1981 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
2041 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
2101 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
2161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
2221 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
2281 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2341 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
2401 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2461 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2521 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
2581 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
2641 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
2701 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
2761 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
2821 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
2881 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
2941 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
3001 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
3061 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
3121 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
3181 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
3241 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
3301 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
3361 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
3421 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
3481 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
3541 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
3601 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
3661 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
3721 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
3781 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
3841 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
3901 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
3961 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
4021 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
4081 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
4141 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
4201 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
4261 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
4321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
4381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
4441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
4501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
4561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
4621 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
4681 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg a
//
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